In vitro Culture of Several Rice Cultivars

Tissue culture methods have been established to regenerate certain rice (Oryza sativa L) cultivars, but regeneration of the rice cultivars widely grown in Arkansas has not been reported. This study has established an in vitroculture for the rice cultivars \u27Nortai\u27, \u27Starbonnet\u27, \u27Mars\u27, Tebonnet\u27, \u27Newbonnet\u27, and \u27Lemont\u27. Callus was induced in the dark at either 20 or 28 C from dehusked seeds cultured on Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) containing 40 g L^-1 sucrose, 10 g L^1 agar, 0.5, 1.0, or 2.0 mg L^-1 1 2,4-dichlorophenoxyacetic acid (2,4-D) and adjusted to pH 5.7. After four weeks the calli were weighed, transferred onto MS medium containing no 2,4-D, and maintained in a 1 2-h photoperiod (65 uE m^-2 s^-1) at 25 ± 2 C to induce plant regeneration. Callus production was best when cultured on a medium containing 1.0 mg L^-1 2,4-D and incubated at 28 C. Plant regeneration was observed two to four weeks later. The percentage of calli regenerating platlets varied with the cultivar and the callus induction treatment. Callus induction at 20 C on a medium with a 2,4-D level less than 2.0 mg L^-1 enhanced the regenerability of most cultivars. Regenerates were transplanted to soil and grow normally to maturity. This system can be helpful in improving rice cultivars with tissue culture techniques such as somaclonal variant selection and somatic hybridization

Effect of Using Ginger Roots Powder and Pure Allicin in Fattening Awassi Lambs Rations on Productive Performance and the Accessory Cut Parts of Carcasses

   أُجري البحث بهدف دراسة تأثير إضافة الاليسين أو الزنجبيل أو خليطهما إلى علائق تسمين الحملان العواسية في الصفات الإنتاجية وبعض الأجزاء الثانوية للذبائح.     استعمل في البحث 28 حملاً عواسياً وزعت عشوائياً على أربعة معاملات متساوية كل معاملة احتوت 7 حملان ، غذيت الحملان على أربعة أنواع من العلائق التجريبية والتي كانت كالآتي: العليقة الأولى عليقة مركزة خالية من الإضافات الغذائية (سيطرة)، العليقة الثانية احتوت على الاليسين النقي بنسبة 3 كغم لكل طن علف مركز، العليقة الثالثة احتوت على مسحوق جذور الزنجبيل بنسبة 2 كغم لكل طن علف مركز أما العليقة الرابعة فقد احتوت على خليط من الاليسين (1.5 كغم/ طن علف مركز) ومسحوق جذور الزنجبيل (1كغم/ طن علف مركز).    غذيت الحملان بطريقة التغذية الجماعية ولمدة 91 يوماً ودرست الصفات الإنتاجية والتي تضمنت الزيادات الوزنية الكلية واليومية ومعامل التحويل الغذائي وكميات العلف المستهلك من الخشن والمركز.    ذبح 12 حملاً من حملان التجربة بواقع ثلاثة حملان من كل معاملة لغرض تسجيل أوزان الأجزاء الثانوية للذبيحة والتي شملت الرأس والجلد والأرجل.    أشارت نتائج البحث إلى عدم وجود فروقات معنوية بين المعاملات المدروسة للأوزان الابتدائية والنهائية والزيادات الوزنية اليومية والكلية، ولم تلاحظ فروقات معنوية في كميات العلف المركز والخشن المستهلك ومعامل التحويل الغذائي، و أوضحت نتائج البحث أيضاً عدم وجود فروقات معنوية في صفة الرأس للذبائح بين المعاملات المختلفة بينما كانت الفروقات معنوية (P<0.05) لصفة الجلد والأرجل.The research has been conducted to investigate the effect of ginger roots powder with allicin in the fattening of Awassi lambs rations with its effect on productive traits as well as the secondary cuts parts of carcasses. A total number of 28 Awassi lambs were used in the research , those lambs were randomly distributed into 7 treatments, lambs fed on 4 experimental rations as follows the first ration was concentrated ration without any supplement (control), the second ration supplemented with 3.0 kg/ton of allicin, the third ration supplemented with 2.0 kg/ton ginger powder, the fourth ration supplemented with a combination of allicin (1.5 kg /ton) and ginger powder (1.0 kg/ton). Lambs fed by group feeding along with the experiment which had been lasted 91 days. The productive traits were studied such as a daily and total weight gain as well as feed conversion ratio and the consumed quantity of concentrated and rough feed. Twenty-one lambs were slaughtered (three lambs for each treatment)  to study the secondary cuts parts of the carcasses. Results revealed no significant differences among treatments in the initial and final weights and daily and total weight gains.  There were no significant differences in the consumed feed among treatments and feed conversion ratio. Results indicated also nonsignificant differences in weights of the head among treatments. Meanwhile, there were significant differences (P<0.05) in skin and legs weights among treatments. &nbsp

Principal Component Analysis Technique for Finding the Best Applicant for a Job

This paper focuses on the use of principal component analysis technique (PCA) in choosing the best applicant for a job in Cihan University-Erbil. Cihan University has a panel of judges (University staff) to help in choosing the applicants for a job by evaluating or rating each one on different scale of preference and different type of characteristics. This process usually creates complicated multivariate data structure, which consists of 25 applicants for a job rated by a panel of judges on 17 characteristics [25 rows, applicants, and 17 columns, characteristics]. PCA plays a crucial role in conducting impactful research as it offers a potent technique for analyzing multivariate data. Researchers can utilize this method to extract valuable information that aids decision-makers in problem-solving. To ensure the appropriateness of data for PCA, certain testing procedures are necessary. In this study, two tests, namely the Kaiser-Meyer-Olkin (KMO) Measure of Sampling Adequacy and Bartlett's Test of Sphericity, were performed, and their significance is vital. The findings indicate that the data employed in this research are suitable for PCA. Scoring and ranking procedures as extra tools were used to see that applicant No. (1) is the first accepted for a job, applicant No. (17) is the second, applicant No. (12) is the third, and so on

Optimum Cone Collection Period in Arkansas for Establishing In vitro Cultures of Loblolly Pine (Pinus Taeda L.)

This study was conducted to determine the optimum period to collect loblolly pine (Pinus taeda L.) cones that contain embryos with the greatest capacity to produce embryogenic callus for establishing somatic embryogenesis. Cones were collected from trees at the University of Arkansas Agricultural Experiment Station, Fayetteville, in 1991 during four consecutive months: May, June, July, and August. Seeds were extracted and disinfected in 70% ethanol for 1 min, 50% Clorox for 20 min, followed by four water rinses. Seed coats were removed, and embryos were cultured on DCR medium adjusted to pH 5.7 and supplemented with 3 mg/L 2,4-D, 0.5 mg/L 6-BAP, 30 g/L sucrose, and 6 g/L agar. Callus was induced and maintained in the dark at 20 ± 5 C. Embryos collected in May failed to proliferate; however, 55%, 88%, and 66% of the embryos cultured produced callus from June, July, and August collections, respectively. Suspension cultures were initiated from pine callus and maintained for over 6 months in MSG medium for subsequent investigation of the conversion of embryogenic complexes into mature embryos and eventually into plantlets. Evidence for embryogenesis was observed with double-staining techniques

Local information pattern descriptor for corneal diseases diagnosis

Light penetrates the human eye through the cornea, which is the outer part of the eye, and then the cornea directs it to the pupil to determine the amount of light that reaches the lens of the eye. Accordingly, the human cornea must not be exposed to any damage or disease that may lead to human vision disturbances. Such damages can be revealed by topographic images used by ophthalmologists. Consequently, an important priority is the early and accurate diagnosis of diseases that may affect corneal integrity through the use of machine learning algorithms, particularly, use of local feature extractions for the image. Accordingly, we suggest a new algorithm called local information pattern (LIP) descriptor to overcome the lack of local binary patterns that loss of information from the image and solve the problem of image rotation. The LIP based on utilizing the sub-image center intensity for estimating neighbors' weights that can use to calculate what so-called contrast based centre (CBC). On the other hand, calculating local pattern (LP) for each block image, to distinguish between two sub-images having the same CBC. LP is the sum of transitions of neighbors' weights, from sub-image center value to one and vice versa. Finally, creating histograms for both CBC and LP, then blending them to represent a robust local feature vector. Which can use for diagnosing, detecting

A Study on Teacher’s Perception about Components of English Handwriting in Pakistan

Handwriting is an essential of school activities for the school going children. Good or legible handwriting remained a constant task for the teachers and students during the whole day. The teachers recognize the legible handwriting at a gland due to their routine work in the schools. The teacher’s criterion that lies behind their decision of good handwriting was a question to answer in this study. The purpose of this study was to identify the components of legibility from the teachers’ perspective. Referencing handwriting experts and a literature review, key variables were categorized and organized onto a 5-point Likert Scale questionnaire. Teachers’ responses to the various legibility criteria were then tallied with regards to primary school students. Mean, standard deviation, exploratory factor analysis and path diagram statistics were applied to the ordinal data. It was concluded that twelve components were important for the legibility of handwriting of primary school students. These include Readability, Margin, Similarity, Line, Space, Size, Shape, Roundness, Form, Slant, Alignment and Recognition

Expression in animal cells and characterization of the hepatitis E virus structural proteins

Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a positive-strand RNA virus with a 7.5-kb polyadenylated genome consisting of three open reading frames (ORFs). In the absence of an in vitro culture system, the replication and expression strategy of HEV and the nature of its encoded polypeptides are not well understood. We have expressed the two ORFs constituting the structural portion of the HEV genome in COS-1 cells by using simian virus 40-based expression vectors and in vitro by using a coupled transcription-translation system. We show here that the major capsid protein, encoded by ORF2, is an 88-kDa glycoprotein which is expressed intracellularly as well as on the cell surface and has the potential to form noncovalent homodimers. It is synthesized as a precursor (ppORF2) which is processed through signal sequence cleavage into the mature protein (pORF2), which is then glycosylated (gpORF2). The minor protein, pORF3, encoded by ORF3 is a 13.5-kDa nonglycosylated protein expressed intracellularly and does not show any major processing. pORF3 interacts with a cellular protein of about 18 kDa which we call 3IP, the pORF3-interacting protein. The significance of these findings are discussed in light of an existing model of HEV genome replication and expression

Bacteriolytic Activity of Coliphages on Diarrhea Associated E. coli

There has been an alarming increase in drug-resistant strains of diarrheagenic Escherichia coli (DEC) in developing as well as developed countries. Several cases of antimicrobial resistance in DEC have been observed in different parts of the world as a result, there has been a renewed interest in alternative antimicrobial treatments, including bacteriophages. This study was conducted to isolation and characterization of a lytic coliphage from sewage water capable to infect a variety of multidrug resistance DEC strains isolated from children suffering diarrhea, as first step to further usage a lytic coliphage in future.in this study, a coliphage was isolated using spotting method and titrated, using agar overlay technique. The host range of coliphages was assessed on a lawn of E coli bacteria. This study included determination of the latent periods and burst size of coli phage then determines the stability of coliphages to physical and chemical condition (temperature, pH and sunlight exposure).The results shown that, five phages isolate (A, B, C, D and E) were exhibiting a potent lytic activity with clear plaques (1-4mm in diameter). Fifty percent of the E coli strains were infected by phage isolates. It seems, very likely, that the coliphages belonging to 3 different groups (1, 2 and3). The phage growth cycle with a detected latent period of 20 min, a burst size of 160 plaque forming units per infected cell, it was found that the phage could survive at varied pH conditions with reduction in its numbers. A temperature of above 60°C and direct sunlight beyond 8 days was found to be deleterious for survival of the phage. Keywords: key words, coliphages, E. coli, diarrhe

On solving fuzzy delay differential equation using bezier curves

In this article, we plan to use Bezier curves method to solve linear fuzzy delay differential equations. A Bezier curves method is presented and modified to solve fuzzy delay problems taking the advantages of the fuzzy set theory properties. The approximate solution with different degrees is compared to the exact solution to confirm that the linear fuzzy delay differential equations process is accurate and efficient. Numerical example is explained and analyzed involved first order linear fuzzy delay differential equations to demonstrate these proper features of this proposed problem

Evaluation of Calvatia Craniformis Mushroom Activity in Treatment of Ringworm Disease Ion Cattle

In order to determine and treatment of the dermatophytosis , that infections in cattle, skin scraping samples were collected from thirty cattle which showed clinical  signs of skin diseases from some farms in different areas in Diyala  governorate. The isolation results in cattle samples showed that (90%) were positive for fungal isolation. The main fungal species that isolated included Trichophyton verrucosum. Three different concentration of Calvatia craniformis mushroom ointment are prepared to obtain (0.25%,0.50% and 1%) of the mushroom ointment respectively and then applied topically to lesion  once daily.Sixty infected cattles in different age and sex were involved in our study  and divided into two  groups ; the first is treatment group and, the second is the control group. The treatment group includes thirty patients  were treated by one of the three concentrations ( Ten patients 0.25% , Ten patients 0.50%, Ten patients 1% concentrations). The control group included thirty patients was treated by 1% sulpher ointment . The 1% mushroom ointment significantly reduce  clearance time  (P<0.05) in comparison with the control group , while other concentration produce non significant changes in clearance time , therefore this substance in all preparations is effective in the treatment of ringworm  . Keywords: ringworm, dermatophytosis, Calvatia craniformi